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Biology, 18.03.2021 01:40 aislynrae22
g Put the following statements in the order they must happen in an experiment to build a plasmid using restriction enzyme-based cloning, some steps might be missing. A. Pick colonies on an LB agar plate with antibiotics B. PCR amplify your DNA of interest, adding restriction enzyme sites to either end C. Transform bacteria (E. coli) to take up DNA plasmid D. Cut vector with restriction enzymes and add DNA insert.
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