Which step in the Gram stain is the critical step in differentiating Gram negative cells from Gram positive cells?

The rapid decolorization with ethanol or acetone

and counterstaining with safranin

When a decolorizer such as alcohol or acetone is added, it interacts with the lipids of the cell membrane. A gram-negative cell loses its outer lipopolysaccharide membrane, and the inner peptidoglycan layer is left exposed: when safranin is added they will turn red. Conversely, gram-positives retain the Cristal violet and will color in purple

protist eukaryotes that are not plants, animals, or fungi ("misfits")

primary succession occurs in essentially lifeless areas regions in which the soil is incapable of sustaining life as a result of such factors as lava flows, newly formed sand dunes, or rocks left from a retreating glacier. secondary succession occurs