In Great Smoky Mountains National Park, about 13 species of fireflies each use light flashes for males and females to recognize each other. The timing and pattern of these flashes is unique for each species. Another group of fireflies does not flash at all and actively mates during the day rather than at
night. This group includes six species.
Identify two modes of isolation observed in related firefly species.
A. Temporal isolation: The species remain separate because the period of most active mating differs.
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B. Geographical isolation: The species remain separate because they have different breeding sites.
C. Behavioral isolation: Each species exhibits a unique flash pattern that allows males and females to recognize each other.
D. Reproductive isolation: Fireflies from different species will not mate with each other because they use different chemical signals.