Use the following to answer questions 34 & 35. A scientist wants to determine whether his favorite protein is an ER resident protein or a cytosolic protein. To address this he conducts the following experiment i) he mixes the mRNA encoding the protein with ribosomes in a cell lysate that contains all the machinery required to support translation as if it were occurring in a cell
ii) he then adds ER derived microsomes to the reaction
iii) he waits for translation to be completed
iv) he then adds proteases to the mixture
v) the purifies his favorite protein out of the mix; he calculates from the DNA sequence that the protein is likely to 500 aa long
34) Assuming that the protein is an ER lumenal protein
a) the protein he purifies is 480 aa long, and cannot be degraded by adding exogenous proteases to his translation mixture
b) the protein he purifies is 500 aa long, and can be degraded by adding exogenous proteases to his translation mixture
c) the protein her purifies is 480 aa long, and can be degraded by adding exogenous proteases to his translation mixture
d) the protein he purifies is 500 aa long, and cannot be degraded by adding exogenous proteases to his translation mixture
e) most of the protein he purifies is degraded by the proteases except ~20 aa which are protected