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An acid-fast staining method was performed on a bacterial smear using the dyes carbol fuchsin and methylene blue. The resulting stained slide contained only cells that appeared blue when visualized using a microscope. Which of the following could explain why only blue cells were present?
a. No acid-fast bacteria are present on the slide
b. Methylene blue was not used
c. Carbolfuchsin was not used
d. Acid-alcohol was not used
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