Aresearcher while working in vitro (outside cell) added helicase enzyme which resulted in the untwisting of the dna helix. but this untwisting led to strain ahead of the replication fork. which enzyme can be added to correct this problem?
nuclease
topoisomerase
telomerase

Option B

Explanation:

Topoisomerase are the enzymes that help in correcting the underwinding of DNA. It act as a maintainer of DNA replication process by preventing the overtightning of the DNA double helix at the time of replication. The DNA helix which is infront of the replication fork is prevented from getting tight at the time of unwinding which is a problem arising due to the double-helical structure of DNA.

Hence, option B is correct

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