In dna sequencing, what stops the replication process?

As helicase unwinds DNA at the replication fork, the DNA ahead is forced to rotate. This process results in a build-up of twists in the DNA ahead.As helicase unwinds DNA at the replication fork, the DNA ahead is forced to rotate. This process results in a build-up of twists in the DNA ahead.This build-up forms a torsional resistance that would eventually halt the progress of the replication fork. Hope this helps