To produce transgenic bacteria that make insulin, which of the steps listed below would a scientist do last?
a. insert the human insulin gene into a plasmid.
b. extract the insulin from the bacterial culture.
c. cut out the insulin gene from human dna.
d. transform bacteria with the recombinant plasmid.
reset selection

poor water quality affects most estuarine organisms, including commercially important fish and shellfish. the pollutants that have the greatest impact on the health of estuaries include toxic substances like chemicals and heavy metals, nutrient pollution (or eutrophication), and pathogens such as bacteria or viruses.

it has to be understood that the sea water contains a huge amount of sodium and chlorine ions and if they enter the cell of the atlantic cod fish, the cell will shrivel and the fish will die. to avoid such a condition, the fish uses active transport for the purpose of removing the ions against the gradient.

explanation:

the atlantic cod is not a maritime osmoconformer. its native application of salts is less than that of the seawater it lives in. it is continually falling water from the gills to the ocean via osmosis.

countering that, the fish is going to be swallowing seawater. but since its internal collection of salts is lower than seawater, the fish will want to go free of excess salt, and since that's going upon a gradient, then active transport, requiring energy, must be used to pump out the salt. much marine fish use the organs to filter out the salt and these fish discharge very straight urine.