In producing a recombinant plasmid to be used to clone a given donor insert, it is possible to cut both the donor and plasmid with the same restriction enzyme, resulting in complementary sticky ends. assuming plenty of plasmid dna is available, why is further selection necessary before the introduction of the plasmid into a cellular system?

I think some donor strands will be inserted with an incorrect orientation. 

Science27 of the most iconic, jaw-dropping photos of the earth and the moon from spacedave mosher  oct 1, 2018, 11: 13 am etnasa/flickrapollo 11 astronauts took this photo of earth on july 20, 1969.from the ground, earth looks like a boundless fertile plain that beckons to be explored and exploited.but astronauts would beg -  and even plead  - to differ."you realize that people often say, 'i hope to go to heaven when i die,'" jim lovell, an astronaut who flew on the apollo 8 and apollo 13 missions to the moon, previously  told business insider. "in reality, if you think about it, you go to heaven when you're born."lovell has seen first-hand that we live on a tiny rock hopelessly lost in the void. he's also quick to tell you it's the only one we've got - a  fragile spaceship  for more than 7 billion people: "you arrive on a planet that has the proper mass, has the gravity to contain water and an atmosphere, which are the very essentials for life. and you arrive on this planet that's orbiting a star just at the right distance - not too far to be too cold, or too close to be too hot - and just at the right distance to absorb that star's energy and then, with that energy, cause life to evolve here in the first place. in reality, you know, god has really given us a stage, just looking at where we were around the moon, a stage on which we perform. and how that play turns out is up to us, i guess."humanity has recorded photos of earth from hundreds, thousands, millions, and even billions of miles away, some of them taken by lovell himself.these images not only scientists study our dynamic world, but also understand how a habitable planet looks from afar, which aids the search for  more worlds. most importantly, however, the images underscore our peculiar existence on  a mote of cosmic dust.take a moment to ponder 27 of the most arresting images of earth that humankind has ever captured from space.a

Answer:

it provides an alternative energy pathway for the reaction to proceed.

explanation:

a catalyst is a substance that speeds up the rate of a chemical reaction but is not consumed during the course of the reaction.

catalysts increase the rate of reaction without being used up. they do this by lowering the activation energy needed. with a catalyst, more collisions result in a reaction, so the rate of reaction increases. different reactions need different catalysts.

a catalyst works by providing an alternative reaction pathway to the reaction product. the rate of the reaction is increased as this alternative route has a lower activation energy than the reaction route not mediated by the catalyst.