Initial velocities have been measured for the reaction of a-chymotrypsin with tyrosine benzyl ester [S] at six different substrate concentrations. Use the data below to make a reasonable estimate of the Vmax and Km value for this substrate. mM [S] 0,00125 0,01 0,04 0,10 2,0 10
V0 (mM/min) 14 35 56 66 69 70

the principles and empirical processes of discovery and demonstration considered characteristic of or necessary for scientific investigation, generally involving the observation of phenomena, the formulation of a hypothesis concerning the phenomena, experimentation to test the hypothesis, and development of a conclusion that confirms, rejects, or modifies the hypothesis.

nb

explanation:

kj

a) carbon, hydrogen

b) carbon, hydrogen, oxygen, nitrogen

c) carbon, hydrogen, oxygen, nitrogen, phosphorus

d) sugar

e) amino acid

f) nucleotide