1. How much biomass did the pansy plant lose after three days? (Hint: Subtract day 3 wet/dry mass from day 1 wet/dry mass. Then multiple this value by the biomass (not water) percentage you calculated in your primary productivity worksheet.) 2. How much energy does the biomass in question 1 represent? (Hint: Each gram of biomass represents about 4.35/g kcal of energy.)

3. What was the total dry mass lost as feces (frass) after three days? How much energy did this frass represent? (Hint: Each gram of frass biomass represents about 4.75 kca/gl of energy.)

4. Calculate the assimilation of primary production by subtracting the energy excreted (lost) by all pansyworms in feces from the total chemical energy consumed by the pansyworms. (Hint: energy consumed by the pansyworms = energy lost by the pansy plant.)

5. Calculate the net secondary productivity of the biomass gained by all the pansyworms in the three-day period of growth. (Hint: Each gram of larvae (pansy worm) biomass represents about 5.5 kcal/g of energy.)

6. Determine the production efficiency between the pansy plant (producer) and pansyworms (consumer) using the production efficiency formula below.

7. Explain why your production efficiency meets or does not meet the expected energy transfer percentage from one level to another.