subject
Biology, 01.05.2021 01:30 herchellann302

The genome of a newly discovered bacteriophage is a linear DNA molecule 10,500 nucleotide pairs in length. One sample of this DNA has been incubated with restriction enzyme X and another sample with restriction enzyme Y. The lengths (in thousands of base pairs) of the restriction fragments produced by the two enzymes have been determined by gel electrophoresis to be as follows: Enzyme X: Fragment X1 = 4.5; X2 = 3.6; X3 = 2.4
Enzyme Y: Fragment Y1 = 5.2; Y2 = 3.8; Y3 = 1.5

Next, the fragments from the enzyme X reaction are isolated and treated with enzyme Y, and the fragments from the enzyme Y reaction are treated with enzyme X. The results are as follows:

X fragments treated with Y: X — 1 → 4.5 (unchanged)
X − 2→2.1 + 1.5
X − 3→1.7 + 0.7
Y fragments treated with X:Y − 1→4.5 + 0.7
Y – 2→2.1 + 1.7
Y − 3→1.5 (unchanged)

Required:
Draw a restriction map of the phage DNA, indicating the positions of all enzyme X and enzyme Y restriction sites and the lengths of DNA between them.

ansver
Answers: 2

Another question on Biology

question
Biology, 22.06.2019 05:00
Identify the examples in which sam interacts only with natural resources
Answers: 2
question
Biology, 22.06.2019 06:30
How does the diagram explain ocean currents? earth tilt 23.5 degreesquestion 1 options: earth's tilt causes uneven heating of earth which causes currents.earth's tilt causes the ocean to move because of gravity.earth's tilt and its rotation cause currents.earth's tilt causes uneven distribution of salt which causes currents.
Answers: 1
question
Biology, 22.06.2019 06:30
Explain how scientists use geologic time to determine the age of landforms.
Answers: 1
question
Biology, 22.06.2019 09:30
Which statement is a physical property? becomes moldy quicklyeasy to digestconducts electricity poorlydoes not burn
Answers: 1
You know the right answer?
The genome of a newly discovered bacteriophage is a linear DNA molecule 10,500 nucleotide pairs in l...
Questions
question
Geography, 18.10.2019 13:30
question
History, 18.10.2019 13:30
question
Mathematics, 18.10.2019 13:30
Questions on the website: 13722367