A. Calculate how to make 10 ml of a 7.5% methylene blue stock solution (from solid powder). B. Calculate how to make a series of 1:10 dilutions of this stock. Imagine you’ll use
5 ml of each dilution for your experiment. Calculate the final concentrations of each tube.
tube 1: take some stock, dilute it 1:10 with some water in a new tube = #1
tube 2: take some of #1, dilute it 1:10 with some water in a new tube
tube 3: take some of #2, dilute it 1:10 with some water in a new tube
tube 4: take some of #3, dilute it 1:10 with some water in a new tube
C. First, decide the total volume to make of each tube – keep in mind measurement accuracy (consider preparing 20% more solution than what you will use).
D. Then, calculate how much stock, water and final concentration of each.
E. Then, instead of doing a serial dilution, calculate making each of the above solutions using the weight/volume % solution method.
F. Pros and Cons of serial dilution method vs W/V % method