An estuary is an area at the mouth of a river where the river joins a sea. Estuaries contain brackish water, which is a mixture of fresh water and salt water. They are rich in nutrients and support a large number of fish species.
The biodiversity of a particular estuary has decreased due to human activities such as pollution, overfishing, and
construction. A scientist wants to develop a method to restore the biodiversity of this estuary. Which method should
the scientist choose?

we solve the problem using the hardy-wineberg equation.

let us start with the homozygous recessive proportion of individuals with sickle cell anaemia,

we have the proportion as q² = 0.25.

∴ q = 0.5 (frequency of hbs in population)

further,

solving for the frequency of normal cell by using the fact that,

p + q = 1

∴ p = 1 - 0.5 = 1 - 0.5 = 0.5

now we can calculate 2pq in p² + 2pq + q², which is the frequency for heterozygous carriers of sickle cell anaemia,

∴ 2pq = 2 x 0.5 x 0.5 = 0.5

and the homozygous normal cell individuals proportion is given by the expression,

∴ p² = 0.5² = 0.25

hence, the carriers for sickle cell anaemia are in proportion, 0.5, or 50%.

the right option is; decreased genetic variation  

decreased genetic variation describes the effect of this environmental change.

genetic variation is the naturally occurring genetic diversities or differences between organisms of the same species in a population. genetic variation allows the survival and adaptability of a species when they encounter changing environmental conditions. when an endangered species reduces, it loses genetic variation which may not be restored even if the species rebound and will only be recovered through the gradual collection of mutations over many years.