subject
Biology, 07.05.2021 18:40 felipeee4609

You are working in a virology lab and are given a sample of high-titer influenza type A virus and Madin-Darby canine kidney (MDCK) epithelial cells which you seed into tissue culture plates. After culturing the MDCK cells to obtain confluent monolayers, your task is to determine the virus titer and express this titer in pfu/1000 microliters. You therefore make four 100-fold dilutions and one 10-fold dilution of the neat virus stock and plate 500 microliters of the final dilution. After 72 hours, you remove the agar overlay, fix and then stain the infected cells with crystal violet. The plates corresponding to your final dilution above contain an average of 27 plaques. What is the titer of the original virus stock

ansver
Answers: 3

Another question on Biology

question
Biology, 21.06.2019 20:30
What is the phylum of soft bodied animals with a true bosy cavity and a three part body consisting of a foot visceral mass and a mantle
Answers: 1
question
Biology, 22.06.2019 06:30
To sciences do not agree on which type of grocery bag is better for the environment what is most likely to come out come out of the disagreement
Answers: 3
question
Biology, 22.06.2019 18:00
Deserts (especially true deserts) are not easy places for animals to live. animals who live in them often have special features that them survive. which adaptation would be for this group of animals? a) keen eye sight b) short, narrow legs c) tolerate heat d) webbed feet
Answers: 2
question
Biology, 22.06.2019 18:00
The images show two species of tree frogs in a particular region. the gray tree frogs are adapted to the trees of the woodlands, while the green tree frogs can survive in woodlands or grasslands. the gray frog was abundant in the woodlands. because of climatic changes, most of the trees in the region died, and the woodlands changed to grasslands. in such a case, the . the gray tree frog is closely related to the green tree frog genetically, but they have different mating calls. this suggests that speciation occurred because of .
Answers: 1
You know the right answer?
You are working in a virology lab and are given a sample of high-titer influenza type A virus and Ma...
Questions
question
Mathematics, 09.04.2021 20:40
question
English, 09.04.2021 20:40
question
World Languages, 09.04.2021 20:40
question
Mathematics, 09.04.2021 20:40
question
Spanish, 09.04.2021 20:40
question
Mathematics, 09.04.2021 20:40
Questions on the website: 13722361