Transformation efficiency is expressed as the number of antibiotic-resistant colonies per μg of plasmid DNA that was actually spread on the plate (colonies/ μg). Determine the transformation efficiency of your culture plate. The most challenging part of this determination is knowing the mass of plasmid actually spread. The concentration of plasmid we used in this experiment was 0.005 μg/ml. Show all work below. # of colonies4 0. 005 N3/me onie Nant: need volume Ma of Plas m lo Lab 6-5 12. Next, completely empty the contents of the eplasmid" tube into a wate beaker and then plectte approximately 200 ul in each tube. Replace both tubes onice. 458 cotonies L O 800 OIO N colonies ee