You are trying to program E. coli bacteria to generate N-acylethanolamide, lipids that have been found to help suppress appetite. In order to do this, you need to transfer the gene for the enzyme N-acyltransferase from the plant Arabidopsis Thaliana into the E. coli bacteria. The transgene, which codes for , will also need to have a
for RNA polymerase and a that would fluoresce to let you know the insertion was successful. You would also need a , a small circular piece of bacterial DNA.
Then you would hunt for a that would cut the plasmid once and also cut the transgene out from its host.

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