When a protein denatures, which type of bonding is effected?

the cladogram shown here shows similarities and differences among several classes of vertebrates. anatomically, the four have limb structure as their common ancestor. we might separate the mammals from the other three classes because they a) divergent; do not lay eggs b) identical; are warm-blooded c) varied; a four chambered heart d) homologous; have mammary glands

well first you need the sample of the temperature and gas level. i couldnt write a hypothesis unless i had some data. i think temperature changes will effect volumes of gas because gas changed into a solid or liquid at specific temperatures.