Biology, 08.07.2021 01:00 sylviaelizabeth4135
Complete the following table to prep your DHFR fusion protein sample for SDS-PAGE. You are looking to load 15ug in a total volume of 40uL.
Protein Concentration (ug/uL)= 1.15ug/ul
Concentration of sample + 2X Laemmli (ug/uL) (Bringing Laemmli to 1X) =
Volume (uL) of sample+Laemmli mix needed for 15ug =
Volume (uL) of 1X Laemmli solution to bring total vol to 40ul=
Answers: 3
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Complete the following table to prep your DHFR fusion protein sample for SDS-PAGE. You are looking t...
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