Lab CUA
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are used
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drug giver
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WBC
Experimental Design and Data Analysis
1. A fearcher would like to stndy the effects of Granulocyte Colony Stimulating Factorte-est) on 32D cells
, a
murine hematopoietic cell line. What does this statement mean?
to grow is
hered
more
formation of blood calls
2. In the laboratory, the researcher begins by using varying concentrations of G-CSF to treat 32D cells for various
periods of time. Why would the researcher have to do this?
3. Below are some results. Explain why the researcher repeated the experiment 3 times.
mo
mD
100
WT
100
100
10-
10
Fold Increase
Fold Increase
Fold increase
6
Time (days)
Time (days)
Time (days)
Cell proliferation/survival analysis of 32D clones using 100 (). 10 (), 1 ( * ), and 0.1 () ng/ml G-CSF.
WT (wild type), mD and mO are G-CSF-R mutants.
4. What can you conclude from these data?
5. Compare and contrast the data from this experiment below.
1000
100
Cell Count (x 10$/mL)
10
1
0.1
0 1 2 3
4 (days)
Parental 32Dc13 cells (solid symbols) and 32D/ɛ cells (open symbols) maintained in IL-3 were washed with PBS
and transferred to medium containing 25 U/mL IL-3 (circle), 50 ng/mL G-CSF (square), or no cytokines (triangle).
Viable cells were counted using the trypan blue dye exclusion method.

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hypoxemia refers to a below usual concentration of oxygen in the blood, mainly in the arteries. hypoxemia is an indication of an issue associated with circulation or breathing and may lead to various symptoms like shortness of breath.  

the condition is identified by determining the level of oxygen in the blood sample obtained from an artery. it can also be predicted by determining the oxygen saturation of the blood with the application of a pulse oximeter.  

the normal arterial oxygen is about 75 to 100 mm hg. the values underneath 60 mm hg is generally considered with the requirement for supplemental oxygen. the normal pulse oximeter readings generally range between 95 to 100 percent. the values under 90 percent are regarded as low.  

in the given case, the oxygen saturation is measured at 93 percent, hence, there is no need to administer oxygen, that is, one should withhold oxygen.  

measuring physical properties lab reportinstructions: in the physical properties lab, you will test the physical properties of different building materials in order to pick the appropriate materials for a new home in tiny world. record your observations and test measurements in the lab report below. youwill submit your completed report.name and title: lyric lawton, mr.herbert, 10/13/17, physical properties lab. objective(s): i need to parvus build his house out of the right material, so he will stay cool in the mountains.hypothesis: if we use wood to build the roof of the house, then the temperature inside the house will remain cooler.if we use clay brick to build the walls and floors of the house, then the temperature inside the house will remain cooler.if we use aluminium to bring electricity into the home, then it will allow electricity to flow into the home at a faster rate.if we use iron to construct the latches on the windows and doors, then the magnetism will keep the latches secure.