Below is the sequence of the HEXA gene with the underlined portion the 1278TATC allele that recessively caused Tay-Sachs disease. Answer the following questions about this sequence: 5’ 3’
3’ 5’

Required:
a. Identify all the PAM sites by clearly marking them in the sequence above.
b. Pick one that is appropriate for the goal of removing the 4 extra TATC nucleotides. There are multiple good answers and you should only choose one.
c. Design a 20bp sgRNA spacer sequence that will make an appropriate cut. There are multiple good answers, and you should only choose one. Be sure to label 5’ and 3’ and the side that would attach to the scaffold sequence.
d. Would you use an HDR template or NHEJ to consistently make the correct edit? Explain

Considering the yellow and green pea color phenotypes studied by gregor mendel: a. what is the biochemical function of the protein that is specified by the gene responsible for the pea color phenotype? (1 point) b. a null allele of a gene is an allele that does not specify (or encode) any of the biochemical function that the gene normally provides (in other words, either no protein at all or only non-functional protein is produced from it). of the two alleles, y and y, which is more likely to be a null allele? (1 point) c. in terms of the underlying biochemistry, why is the y allele dominant to the y allele? (2 points) d. why are peas that are yy homozygotes green? (1 point) e. the amount of protein produced from a gene is roughly proportional to the number of functional copies of the gene carried by a cell or individual. what do the phenotypes of yy homozygotes, yy heterozygotes, and yy homozygotes tell us about the amount of sgr enzyme needed to produce a yellow color? explain your reasoning. (2 points)

Asap what occurs after cytokinesis is completed at the end of meiosis 1?

What is the farthest destination to date for a human spaceflight mission?