Locate the positions were EcoRI, EcoRV, Nsil, and BamHI cut the plasmid. Will these enzymes cut on both sides of the insert to cut it back out? what will happen if we use an enzyme that cuts at only one site?

primary succession occurs in essentially lifeless areas regions in which the soil is incapable of sustaining life as a result of such factors as lava flows, newly formed sand dunes, or rocks left from a retreating glacier. secondary succession occurs

during the process of dna replication, brdu can replace thymidine and incorporate into the newly synthesized dna, cell proliferation thus can be reflected in the extent of brdu incorporation.

idk im sorry.