Answer my question here for my watch silence class

Protein and polypeptide sequences can be determined by first partially hydrolyzing the protein or polypeptide followed by amino acid sequencing of the fragment pieces using the chemical degradation process known as edman degradation. determine the sequence of the initial (18-amino acid) polypeptide that produces the following five partial sequences after hydrolysis and edman degradation.

Assume that you are interested in separating short (200-400 bp) dna molecules from a pool of longer molecules in the 10,000-20,000 nucleotide range. you have two recipes for making your polyacrylamide gels: one recipe uses 1.5 percent agarose and would be considered a “hard gel,” while the other uses 0.5 percent agarose and would be considered a loose gel. which gel should you use to separate the short (200-400 bp) dna molecules from a pool of longer molecules in the 10,000-20,000 nucleotide range?

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