The two strands that make up the DNA double helix are said to be antiparallel. What does that mean? Nucleotides containing adenine base pair with nucleotides containing thymine.

The 5' to 3' direction of one strand runs counter to the 5' to 3' direction of the other strand.

Base pairings create unequal spacing between the two DNA strands.

The 5' to 3' direction of one strand runs counter to the 5' to the 3' direction of the other strand.

Explanation:

Antiparallel strands of a DNA molecule means that the phosphate groups at the start of two DNA strands are in opposite position (pole).

The DNA molecule is composed of two strands held together by hydrogen bonds. A single strand is different at its two ends. One end is called 5' (5 prime), the other is called 3' (3 prime). The names come from the notation for the two sugar carbon atoms which participate in the phosphodiester bonds. The different strands in the helix run in opposite (anti-parallel) directions. At each end of the double helix, one strand is 5' and the other is 3'.

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