QUESTION 11 2.703 points
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Lacy had set up two enzymatic reactions using catechol oxidase: Sample #1 contained catechol
oxidase and hydroquonine, and sample #2 contained catechol oxidase and catehol.
Explain these absorbance values:
Sample: Absorbance Value:
#1
0.0034
#2
1.987
a. Hydroquinonone is the best substrate for catechol oxidase as it yields the highest amount
of benzoquinone.
b. Hydroquinone and catechol are structurally the same.
c. Enzymes are substrate-specific. Catechol oxidase binds best with catechol due to it's
structure, ana yields a high amount of benzoquinone.
d. Hydroquinone fits well in the active site of catechol oxidase, but is released from the active
site before the catalytic reaction occurs.
e. Hydroquinone and catechol are equally compatible substrates for catechol oxidase.
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