2. List 5-7 possible factors/conditions that should be tested through repeated trials when designing an optimal activity assay for an enzyme. Choose 2 items from your list and explain how you would optimize them for your POI.

the oldest known insects are believed to live around 400 millions ago as suggested by the fossil records.   some plants having woody stems also evolved during this time.

after the evolution of insects the first four legged species or the tetrapods evolved in shallow freshwater habitats from the intermediate species approximately 397 million years ago. the tetrapods went on conquering the land giving rise to amphibians, reptiles, mammals and birds.

the major split in tetrapods first occurred 340 million years ago branching off from amphibians.

363 million years ago when the carboniferous period began the insects started roaming on land and taking flights, following which the four limbed tetrapods adapted in the terrestrial habitat.

osteogenesis imperfecta (oi) refers to a congenital ailment of bones. in the condition, the bones become brittle or fragile. this ailment is a general result of the defect or mutations in the genes (colia1 or colia2). this mutation results in imperfect collagen formation either in quantity or quality wise.  

there are eight kinds of oi defects, and each kind varies with certain distinctions in symptoms. the usual symptoms include frequent bone fractures, loss of hearing in infants, protruding eyes, discoloration of the white in the eyes, respiratory issues, loosening of the joints, short stature, and other symptoms.