g You want to study a eukaryotic protein in a cell-free reaction system (just protein in buffer), so you first need to purify your protein. You clone the gene and its promoter region, then incorporate it into a plasmid. After transforming the plasmid into bacterial cells, you note that general protein expression has initiated but your protein is not observed after homogenizing the cells and analyzing the lysate. Why might this be true and how would you resolve it

just here for the other one bc they put up with u

estuaries are the partially enclosed coastal bodies of brackish water formed at the mouth of sea where river meets an ocean. it is a transition zone between river and sea water so estuaries are influenced to both marine environment such as tides, waves and to riverine such as flow of fresh water and sediments. the mixing of sea and fresh water provide high level of nutrients, making estuaries a high product habitats.

many estuaries suffers degradation from a variety of human activities like erosion from deforestation, overgrazing, overfishing, drainage and eutrophication.

so from the above statement answers will be

1. false as estuaries are formed due to both brackish and freshwater.

2.true, due to high productivity estuaries are breeding ground for many marine species.

3. direct and indirect activities of man that contribute to pollution and deposition of sediments.

blood enters the heart through two large veins, the inferior and superior vena cava, emptying oxygen-poor blood from the body into the right atrium of the heart. as the ventricle contracts, blood leaves the heart through the pulmonic valve, into the pulmonary artery and to the lungs where it is oxygenated.