In two-dimensional electrophoresis Question 3 options: Proteins are separated by native size with molecular sieve electrophoresis. Then the proteins are separated by their isoelectric point with isoelectric focusing. Denatured proteins are separated by subunit size with denaturing SDS electrophoresis. Then native proteins are separated by their isoelectric point with isoelectric focusing. Native proteins are separated by their isoelectric point with isoelectric focusing. Then they are separated by subunit size with denaturing SDS electrophoresis. Native proteins are separated by their charge with isoelectric focusing. Then they are separated by protein size by denaturing SDS electrophoresis. Native proteins are separated by protein size with SDS electrophoresis. Then denatured proteins are separated by their charge with isoelectric focusing.

To answer this question, researchers studied populations of the dusky salamander (desmognathus ochrophaeus) living on different mountain ranges in the southern appalachian mountains. the researchers tested the reproductive isolation of pairs of salamander populations by leaving one male and one female together and later checking the females for the presence of sperm. four mating combinations were tested for each pair of populations (a and b)—two within the same population (female a with male a and female b with male b) and two between populations (female a with male b and female b with male a). the proportion of successful matings for each mating combination was measured. for example, when all the matings of a particular combination were successful, the researchers gave it a value of 1; when none of the matings were successful, they gave it a value of 0. then the researchers calculated an index of reproductive isolation that ranged from 0 (no isolation) to 2 (full isolation). the reproductive isolation value for two populations is the sum of the proportion of successful matings of each type within populations (aa + bb) minus the sum of the proportion of successful matings of each type between populations (ab + ba). the table provides data for the geographic distances and reproductive isolation values for 27 pairs of dusky salamander populations.

80 points pls brainliest 1. what does a red shift mean? blue shift? 2. describe the big bang theory. according to this theory, how old is the universe? 3. scientists believe the universe is expanding. what is the evidence that supports this? 4. describe a nebula. 5. describe the 3 types of galaxies. what is a barred spiral galaxy? 6. what is a light year? how far is alpha centauri from earth? 7. describe the universal law of gravitation. be sure to include gravitational force between two objects. 8. describe the planets’ orbits around the sun. 9. what is the asteroid belt? where is it located? 10. describe rotation and revolution of earth. what determines an earth day and year? 11. how do galaxies exist? 12. compare and contrast the inner and outer planets. 13. what causes the seasons?

If a cell is placed into a hypertonic environment,what will happen to it ? a)it will shrink or shrivel b)nothing c)it will burst d) it will expand or enlarge