Suppose you had poured iodine on your plate and noticed clearings in the uninoculated area, as well as around both of your transferred cultures?

If after pouring iodine on an uninoculated plate clearings as well as in the transferred plates it means that
1. THE EXPERIMENT WAS NOT CONDUCTED PROPERLY.
2. THE UNINOCULATED PLATE WAS CONTAMINATED AND SHOULD BE DISCARDED.
3. THE CULTURE MEDIUM WAS NOT PREPARED PROPERLY BECAUSE THE STARCH WAS NOT MIXED WELL.
4. THE EXPERIMENT SHOULD BE DONE AGAIN WITH PRECISION AND CARE.

Mitochondria produce atp, a high energy compound. atp is used for all sorts of enzyme-mediated reactions, such as making proteins/nucleotides/etc, certain compounds cross the cell membrane, muscles move, etc.  without this source of energy, a cell will die. it will not be able to repair itself, defend against pathogens, etc.