Plant cells have a cell wall, which means that gene transfer can be done only by physical methods. which method would be preferred for the production of a pesticide–resistant plant?
gene transfer using a plasmid from e. coli
gene transfer using a bacteriophage
gene transfer using a gene gun

Gene transfer using a gene gun. This is the second method of gene transfer in plants. The so-called gene gun fires gold particles containing foreign DNA into the cells of the plants. Few of these particles can pass through the cell wall and reach the cell nucleus where the gene can integrate itself into the chromosomes.