Part b - irreversible inhibition you have added an irreversible inhibitor to a sample of enzyme and substrate. at this point, the reaction has stopped completely. what can you do to regain the activity of the enzyme? view available hint(s) what can you do to regain the activity of the enzyme? removing the irreversible inhibitor should get the reaction working again. the enzyme is inactive at this point. new enzyme must be added to regain enzyme activity. adding more substrate will increase the rate of reaction. adding more inhibitor should get the reaction up to speed again.