Chemistry, 10.09.2019 18:30 keirarae2005
1. separation of amino acids by ion-exchange chromatography. mixtures of amino acids can be analyzed by first separating the mixture into its components through ion-exchange chromatography. amino acids placed on a cation-exchange resin containing sulfate (-so3-) groups flow down the column at different rates because of two factors that influence their movement (1) ionic attraction between the sulfonate residues on the column and positively charged functional groups on the amino acids, and (2) hydrophobic interactions between amino acid side chains and the strongly hydrophobic backbone of the polystyrene resin. for each pair of amino acids listed, determine which will be eluted first from the cation-exchange column by a ph 7.0 buffer.
a.) asp and lys
b.) arg and met
c.) glu and val
d.) gly and val
e.) ser and ala
Answers: 2
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1. separation of amino acids by ion-exchange chromatography. mixtures of amino acids can be analyzed...
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