Dilution question in many of the experiments, you will be asked to prepare a standard solution by diluting a stock solution. you will be specifically asked how you will do the dilution steps during the review before you start work. here is a homework question to get you to think about this process. you have a 1000 ug/ml stock solution of lead (pb2+). describe how you would prepare a 100 ppb standard solution from this stock solution. rules: no single dilution step can be greater than 1: 50 you have 25.00 ml volumetric flasks and 10.00 ml volumetric flasks. you have access to three pipet dispensers. one dispenser delivers between 1 and 5 ml, the second dispenser delivers between 100 and 1000 , and the third dispenser delivers between 20 and 200 hl

We could do two 1:50 dilutions and one 1:4 dilutions.

Explanation:

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A solution that is 1000 ug/ ml  (or 1000 mg / l) is 1000 ppm.

Knowing that 1 ppm = 1000 ppb, 100 ppb is 0.1 ppm.

Then, we have to dilute the stock solution (1000 ppm / 0.1 ppm) 10000 times.

We could do two 1:50 dilutions and one 1:4 dilutions (50 · 50 · 4 = 10000). Since the first dilution is 1:50, you will use the smallest quantity of the stock solution (if we use the 10.00 ml flask):

First step (1:50 dilution):

Take 0.2 ml of the stock solution using the third dispenser (20 - 200 ul), and pour it in the 10.00 ml flask. Fill with water to the mark (concentration : 1000 ppm / 50 = 20 ppm).

Step 2 (1:50 dilution):

Take 0.2 ml of the solution made in step 1 and pour it in another 10.00 ml flask. Fill with water to the mark. Concentration 20 ppm/ 50 = 0.4 ppm)

Step 3 (1:4 dilution):

Take 2.5 ml of the solution made in step 3 (using the first dispenser 1 - 5 ml) and pour it in a 10.00 ml flask. Fill with water to the mark. Concentration 0.4 ppm / 4 = 0.1 ppm = 100 ppb.

a  solution  is always transparent, light passes through with no scattering from solute particles which are molecule in size. the  solution  is homogeneous and does not settle out. a colloid is intermediate between asolution  and a  suspension. while asuspension  will separate out a colloid will not.

in 1783 cavendish was working with his hydrogen gas and found that when he burned it and condensed the vapor it was water. this was very significant since it disproved the greek theory of elements completely since water was not a simple substance but the product of two gases. it was lavoisier who hearing of the experiment gave cavendish's gas the name hydrogen ("water-producer") and added that hydrogen burned by combining with oxygen and that water was a hydrogen-oxygen combination.

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