400 mm nacl.400 mm nacl. a 1 ml1 ml sample of the protein solution is placed in a tube made of dialysis membrane and dialyzed against 2.0 l2.0 l of the same hepes buffer with 0 mm nacl.0 mm nacl. small molecules and ions, such as na, na, cl, cl, and hepes, can diffuse across the dialysis membrane, but the protein cannot. once the dialysis has come to equilibrium, what is the concentration of naclnacl in the protein sample? assume no volume changes occur in the sample during the dialysis. [nacl]=[nacl]= if the original 1 ml1 ml sample were dialyzed twice, successively, against 100 ml100 ml of the same hepes buffer with 0 mm nacl,0 mm nacl, what would be the final naclnacl concentration in the sample? [nacl]=[nacl]=

The answer to your question really depends on three things: how much water you have, how cold it is to start out, and how cold the things around it are. water actually freezes when it gets to 32 degrees fahrenheit (0 degrees celsius), but the time it takes to get there may be different.  let's start with the first. if you take two glasses, and fill one with a tiny bit of water, and the other about halfway, then put them both in the freezer, the one with less water will freeze first (you can try this at home, but i recommend using plastic cups and not glass ones).  now let's move on to the second part. let's say you have two glasses, and you fill one with really cold water that has been in the refrigerator, and the other with really hot water from the sink. if you put both of them in the freezer, the one that started out colder will freeze first.  for the third part, let's imagine that you have two glasses with the same amount of water in them, and the water is at the same temperature. imagine putting one outside on a really really cold day in georgia, and having a friend in alaska put one outside on the same day. since it would be so much colder in alaska, the glass of water there would freeze before yours.  so, if you took a tiny bit of really cold water in a glass, and put it outside on a cold day in alaska, it would freeze a lot faster than a big glass of hot water outside on a cold day in georgia. 

The answer is neonhope it