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Chemistry, 10.10.2019 02:00 aesthvx44

The hydrolysis of pyrophosphate to orthophosphate drives biosynthetic reactions such as dna synthesis. in escherichia coli, a pyrophosphatase catalyzes this hydrolytic reaction. the pyrophosphatase has a mass of 120 kda and consists of six identical subunits. a unit of activity for this enzyme, u, is the amount of enzyme that hydrolyzes 10 μmol of pyrophosphate in 15 minutes. the purified enzyme has a of 2800 u per milligram of enzyme. when [s]> > km, how many micromoles of substrate can 1 mg of enzyme hydrolyze per second?

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