For her undergraduate project, jessica studied an enzyme that catalyzes the reaction a = b. for substrate a, she determined that km = 4.5 μm and kcat = 35 min–1. jessica graduated and her project has been passed on to you. unfortunately, jessica was so busy that she sometimes forgot to record all of the details of an assay in her lab notebook. your mentor suggests that you try to back calculate some of the missing concentration values. assume that the enzyme follows michaelis-menten kinetics. a.) in one experiment where [a] = 5 mm, she found that the initial velocity, v0 was 334 nm min–1. what was the [et] she used in this experiment?