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Chemistry, 16.10.2019 05:10 klbryant2001

In column chromatography (microscale), after loading it with solvent and adsorbent and prior to loading the sample, what level should you allow the solvent to drop to? why should you not allow the sample to run dry and what is the consequence if you had allowed it to do so? why would water not be used to elute a column (besides it polarity) in column chromatography? when isolating separated compounds from a column chromatography, what would aid in the identification of compounds in fractions in the tlc analysis?

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In column chromatography (microscale), after loading it with solvent and adsorbent and prior to load...
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