What would be the order of elution (from first --> last) of the proteins carbonic anhydrase (pi = 7.0), carboxypeptidase b (pi = 6.2) and chymotrypsin (pi = 8.0) run on a cm (carboxymethyl) ion exchange column at ph 6.6 eluted with an increasing nacl gradient:

carboxypeptidase b, carbonic anhydrase, chymotrypsin.
chymotrypsin, carboxypeptidase b, carbonic anhydrase.
carboxypeptidase b and carbonic anhydrase will elute together followed by chymotrypsin.
carbonic anhydrase, chymotrypsin, carboxypeptidase b.
chymotrypsin, carbonic anhydrase, carboxypeptidase b.

What would you do if you told the guy you liked that you liked him

Problem #3 (ch. 1, problem 15)the ideal gas law provides one way to estimate the pressure exerted by a gas on a container. the law isí‘ťí‘ť=푛푛푛푛푛푛푉푉more accurate estimates can be made with the van der waals equationí‘ťí‘ť=푛푛푛푛푛푛푉푉â’푛푛푟푟â’푞푞푛푛2푉푉2where the term nb is a correction for the volume of the molecules and the term an2/v2is a correction for molecular attractions. the values of a and b depend on the type of gas. the gas constant is r, the absolutetemperature is t, the gas volume is v, and the number of moles of gas molecules is indicated by n. if n = 1 mol of an ideal gas were confined to a volume of v = 22.41 l at a temperature of 0â°c (273.2k), it would exert a pressure of 1 atm. in these units, r = 0.0826.for chlorine gas (cl2), a = 6.49 and b = 0.0562. compare the pressure estimates given by the ideal gas law and the van der waals equation for 1 mol of cl2 in 22.41 l at 273.2 k. what is the main cause of the difference in the two pressure estimates, the molecular volume or the molecular attractions?

Modern pennies are composed of zinc coated with copper. a student determines the mass of a penny to be 2.482 g and then makes several scratches in the copper coaling (to expose the underlying zinc). the student puts the scratched penny in hydrochloric acid, where the following reaction occurs between the zinc and the hcl (the copper remains undissolved): zn(s) + 2 hcl(aq) → h2(g) + zncl(aq)the student collects the hydrogen produced over water at 25 °c. the collected gas occupies a volume of 0.899 l at a total pressure of 79 j mmhg. calculate the percent zinc (by mass) in the penny. (assume that all the zn in the penny dissolves.)