Peptides can be separated using an ionâexchange column based on their isoelectric (pi) values. at which ph values would two different peptides, one with a pi of and the other with a pi of , bind to a cationâ and anionâexchange column? each peptide may be capable of binding to each column at more than one ph value.

What is the difference between covalent bonds and ionic bonds? covalent bonds involve the sharing of electrons between atoms; ionic bonds involve the electrical attraction between charged atoms. covalent bonds involve the transfer of electrons between charged atoms; ionic bonds involve the sharing of electrons between atoms. covalent bonds involve the sharing of pairs of electrons between atoms; ionic bonds involve the sharing of single electrons between atoms. covalent bonds involve the sharing of electrons between atoms; ionic bonds involve the sharing of protons between charged atoms.