Afluorescence assay was to be tested to see what range of concentrations it was linear. the following data were obtained for quinine sulfate standards ranging in concentration from 10 down to 10°m. given the wide range to be tested, it was necessary to change the attenuation of the signal to keep the readings on scale. therefore, a blank reading was made at each attenuation level to correct for background signal intensity. the intensities are on a linear scale with an arbitrary multiplier. attenuation log of fluorescence concentration intensity of sample fluorescence intensity of blank 3,500 5,853 5,928 6,099 6,038 6,335 10,000 10,000 10,000 10,000 1,000 100 10 42 390 4003 (a) multiply each standard reading by the attenuation and correct for the contribution found for a blank sample run with the same attenuation setting. (b) plot the corrected intensities vs. concentration on a log-log plot. (e) over what concentration range would the method be useful? since all compounds that fluoresce/phosphoresce also absorb light, a number of analytical samples can be analyzed either by absorption or luminescence spectrometry. when does one consider using luminescence as opposed to absorption methods?