Aresearcher used hplc to examine a bicomponent mixture containing 1.22 mg/l of compound a and 1.31 mg/l of compound b, which was added as an internal standard. this mixture produced peak areas for compounds a and b of 10919 and 5379 , respectively. using the above information, determine the response factor (f).

after establishing f, the researcher prepared a solution by combining 8.18 mg of b with 10.00 ml of an unknown solution containing only a and then diluted it to a final volume of 50.00 ml. the sample was examined using hplc and peak areas of 6065 and 9111 were observed for a and b, respectively.

determine the concentration of a (mg/ml) in the unknown solution.

Response factor A: 8950 L/mg

Response factor B: 4106 L/mg

A = 02497 mg/mL

Explanation:

The response factor (F) is defined as the ratio between the signal produced by an analyte and its concentration.

For A:

F(A) = 10919 / 1,22mg/L = 8950 L/mg

F(B) = 5379 / 1,31mg/L = 4106 L/mg

It is possible to obtain relative response factor (RRF) that is the ratio between F(A) and F(B), thus:

RRF = 8950 L/mg / 4106 L/mg = 2,180

RRF could be:

That is:

The concentration of B in mg/mL is:

8,18mg / (10,00mL + 50,00mL) = 0,1363 mg/mL

Replacing:

A = 0,04162 mg/mL -In the diluted solution-

The concentration in the unknown solution is:

0,04162 mg/mL × = 02497 mg/mL

I hope it helps!

each  column  of the  table  is called a group. elements in a group share similar properties. groups are  read  from  top  to  bottom. metal. metalloid. nonmetal. solid. liquid. gas. fe. hg. o.  2. 3. 4. 5. 6. 7. 8. 9.  2. beryllium. 9.012. 4.  sodium. 22.990.  11. 3.  magnesium. 24.305.  12.  potassium. 39.098.  19. 4.  calcium. 40.078.  20. i guess  i  

the transfer of heat by electromagnetic radiation is called thermal radiation . it is usually carried out by electromagnetic waves in an energy range just below the visible spectrum, which is the infrared range.

so the answer would be radiation.

: )