Using the molecular weight of tris base (121.14 g/mol), calculate how much tris base you need to make 50 ml of a 1.0 m solution. next, using the henderson-hasselbalch equation calculate how much 6.0 m hcl you expect to have to add to make the solution have a final ph of 8.0. use 8.06 for the pka value of tris. 10x tbe: prepare 50 ml of 10x tbe in a conical tube. the concentrations of components of the 10x stock solution are: 0.89 m tris base (121.1 g/mol), 0.89 m boric acid (61.8 g/mol), and 0.020 m edta (0.50 m stock solution). 2x alkaline phosphatase reaction buffer: with the 1.0 m tris ph 8.0 stock solution you prepared and the provided 0.5 m mgcl2, make a 50 ml solution of 0.40 m tris, ph 8.0 + 10 mm mgcl2. this buffer will be used as the reaction buffer for our experiments on enzyme kinetics. since you're making it as a 2x solution, this means you'll be diluting the buffer 1: 1 (1 part buffer to 1 part other stuff) in doing your kinetics reactions later in the semester. mbp purification buffer: prepare 50 ml in a conical tube. use the provided 1 m tris, ph 7.4 (not the tris ph 8.0 you made earlier), 4 m nacl, and 0.5 m edta as stock solutions to make a buffer with the following composition: 20 mm tris ph 7.4, 200 mm nacl, and 1 mm edta