Affinity chromatography is used to purify a protein of interest by capturing it on select one:

a. matrix material with a strongly interacting molecule (very high dissociation constant with target protein) attached.
b. matrix with very small pores that will only allow the protein of interest to pass.
c. matrix material with a strongly interacting molecule (very low dissociation constant with target protein) attached.
d. matrix material with molecules of opposite charge attached (relying on attraction between positive and negative charges).

c. matrix material with a strongly interacting molecule (very low dissociation constant with target protein) attached.

Explanation:

Affinity chromatography (AC) is based on the immobilization of the protein of interest inside of the column, by means of having a strongly interacting molecule in the matrix.

Strongly interacting means that the complex Protein-Interacting Molecule will not be easily dissociated, this translates into a low dissociation constant.

the answer would be b i believe

the best answer is d because the strong cohesion is caused by the ability of water molecules to undergo hydrogen bonding.

a is incorrect because when water solidifies, it becomes   ice, which expands and occupies a bigger volume for the same mass of water in its liquid state.

b is incorrect because water has high surface tension. besides bonding to itself it hydrogen bonds to other molecules at the top decreasing possibility for breaking of attractive forces.

c is incorrect because the water molecule has oxygen which is highly electronegative and so pulls the electron pair towards itself creating a dipole moment, making the molecule polar.

e is incorrect because water has strong adhesion, ability to stick to other molecules such as glass because of this molecules having stronger hydrogen bonding than water.