How much of a 25mg/ml chloramphenicol stock solution do we need to make 400ml of growth media that has final 25ug/ml concentration of chloramphenicol?

Answer : The volume of chloramphenicol stock solution needed is, 0.4 mL

Explanation :

First we have to calculate the mass of chloramphenicol.

As, the mass of chloramphenicol in 1 mL of solution =

So, the mass of chloramphenicol in 400 mL of solution =

Thus, the mass of chloramphenicol is, 0.01 g

Now we have to calculate the volume of chloramphenicol stock solution needed.

Concentration of chloramphenicol =

As, of chloramphenicol needed 1 mL volume of chloramphenicol stock solution

So, of chloramphenicol needed volume of chloramphenicol stock solution

Thus, the volume of chloramphenicol stock solution needed is, 0.4 mL

indicate valleys, hills, steepness and gentleness of slopes.

explanation:

contour lines on a map indicate elevation, or depth. numeric notations on contour lines refer to the level of elevation, or height about sea level.

58750.08068 is the answer i think