) chromatography is a method of fractionating a protein mixture according to the different polarities of the proteins. A). hydrophobic B). ion exchange column C). salting out D). affinity column E). Gel filtration

Option (A) is the correct answer.

Explanation:

A technique used to physically separate the components of two phases, one phase is stationary and the other phase is moving in a definite direction is known as chromatography.

In chromatography, the moving or mobile phase leaves the column.

A technique which uses properties of hydrophobicity in order to separate the proteins from each other is known as hydrophobic interaction chromatography. In this technique, the protein remains attached to the stationary column. Therefore, it easily gets separated out.

Thus, we can conclude that hydrophobic interaction chromatography is a method of fractionating a protein mixture according to the different polarities of the proteins.

1. they vary with a higher number because boiling is way hotter than melting