Chemistry, 20.02.2020 18:04 inesdelude

Acetylcholinesterase catalyzes the hydrolysis of the neurotransmitter acetylcholine:

Acetylcholine + H2O ? acetate + choline

The Km of acetylcholinesterase for its substrate acetylcholine is 9.0 x 10-5M. In a reaction mixture containing 5 nmol/mL of acetylcholinesterase and 150.0 ?M acetylcholine, a velocity, Vo = 40.0 ?mol/mL*sec was observed for the acetylcholinesterase reaction.

A. Calculate Vmax for this amount of enzyme.

B. Calculate kcat for acetylcholinesterase.

C. Calculate the specificity constant for acetylchoinesterase.

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Acetylcholinesterase catalyzes the hydrolysis of the neurotransmitter acetylcholine:

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