You were instructed to add 1.0 mL out of 5.0 mL of an undiluted sample to 99 mL of sterile diluent. Instead, you add the entire 5.0 mL to the 99 mL. A.) What was your intended dilution factor? (2 points) B.) What was your actual dilution factor? (2 points)

Intended dilution factor =  

and

Actual dilution factor =  

Explanation:

Given;

The intended added volume of the undiluted sample = 1.0 mL

The volume of the sterile diluent = 99 mL

The intended added volume = 5 mL

now,

the dilution factor is given as :

Dilution factor = (Volume added to the solution)/(Total volume of the solution )

thus,

the intended dilution factor =

or

Intended dilution factor =  1.0ml/1+99ml

also,

the actual dilution factor = 100

or 5.0ml/5+99ml

Actual dilution factor = 104