In a particular enzyme, an alanine residue is located in a cleft where the substrate binds. A mutation that changes this residue to a glycine has little effect on activity; however, another mutation, which changes the alanine to a glutamate residue, leads to a complete loss of activity. Provide a brief explanation for these observations.

Answer asap need it by wednesday morning carry out the following calculations on ph and ka of from data. i. calculate the ph of 0.02m hcl ii. calculate the ph of 0.036m naoh iii. calculate the ph of 0.36m ca(oh)2 iv. calculate the ph of 0.16m ch3cooh which has ka = 1.74 x 10-5 mol dm-3 v. calculate ka for weak acid ha which has a ph of 3.65 at 0.30m concentration vi. calculate the ka of a solution made by mixing 15.0 cm3 0.2m ha and 60.0 cm3 0.31m a-. [ph= 3.80] vii. calculate the ph of a solution made by mixing 15.0 cm3 0.1m naoh and 35.0 cm3 0.2m hcooh. [ka = 1.82 x 10-4 m]