Chemistry, 17.03.2020 04:31 elopezhilario4389
PCL was mixed with gelatin to make a blend for elctrospun fibrous scaffold encapsulating growth factor that was admixed in the polymer solution and then filled in the syringe for electrospinning. Three scaffolds were made with (A: only PCL), (B: PCL:gelatin=3:1), (C: PCL:gelatin = 1:1). The scaffolds were immersed in PBS at 37 °C! and its degradation rate was noted: Scaffold A, B and C degraded 1%, 25% and 50% respectively, in 21 days. Assuming the growth factor is trapped and cannot freely diffuse out of the scaffold. Assuming, growth factor release is dependent on scaffold degradation A. Which scaffold can be used for delivering growth factor (encapsulated in the scaffold) needed to be delivered in first 7 days of incubation in an in-vivo experiment and why? (5pts) B. The scaffold A and C were prepared and freeze dried. Their weights were Dry weight of A: 10 mg Dry weight of C: 10 mg Both (A and C) the scaffolds were kept in PBS for 7 days at 37 °C. The scaffolds were freeze dried and their weight was recorded. Weight of A after degradation: 9.967 mg Weight of C after degradation: 8.33 mg Calculate the percentage weight remaining for A and C scaffold and comment on why one scaffold degraded faster than the other.
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PCL was mixed with gelatin to make a blend for elctrospun fibrous scaffold encapsulating growth fact...
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