Estimation of Vmax and Km by Inspection Although graphical methods are available for accurate determination of the Vmax and Km of an enzyme-catalyzed reaction, sometimes these quantities can be quickly estimated by inspecting values of V0 at increasing [S].
Estimate the Vmax and Km of the enzyme-catalyzed reaction for which the following data were obtained.

[S], (M) v0= μ m/min=initial rate
2.5 x 10^(-6) 28
4.0 x 10^(-6) 40
1 x10^(-5) 70
2 x 10^(-5) 95
4 x 10^(-5) 112
1 x 10^(-4) 128
2 x 10^(-3) 139
1 x 10^(-2) 140